How to Reconstitute GHK-Cu

GHK-Cu (Copper Tripeptide-1) is widely studied for skin regeneration and tissue repair research. Proper reconstitution ensures accurate peptide concentration and solution stability.

You can find laboratory-grade GHK-Cu peptides here.

Materials Required

• GHK-Cu vial
• Bacteriostatic water
• Sterile syringe
• Alcohol swabs

Reconstitution Process

1. Sanitize vial tops.
2. Draw bacteriostatic water.
3. Inject slowly along the vial wall.
4. Allow the peptide to dissolve.
5. Gently swirl if needed.

How to Calculate Peptide Concentration After Reconstitution

When a peptide is reconstituted, a known amount of powder (mg) is dissolved in a specific amount of liquid (mL). The goal is to determine how much peptide is contained in each mL of solution.

Formula:
Concentration (mg/mL) = Peptide amount (mg) ÷ Liquid added (mL)

Example:
5 mg peptide + 2 mL liquid
5 ÷ 2 = 2.5 mg/mL

This means each mL contains 2.5 mg of peptide.

Converting mg to mcg

Peptide doses are often measured in micrograms (mcg).

1 mg = 1000 mcg

Example:
2.5 mg/mL × 1000 = 2500 mcg/mL

Finding the Volume for a Dose

To determine how much solution contains your desired dose:

Volume (mL) = Desired dose ÷ Concentration

Example:
Desired dose: 500 mcg
Concentration: 2500 mcg/mL

500 ÷ 2500 = 0.2 mL

Insulin Syringe Conversion

Most peptides are measured using U-100 insulin syringes.

1 mL = 100 units
0.5 mL = 50 units
0.2 mL = 20 units
0.1 mL = 10 units
0.05 mL = 5 units

Example:
0.2 mL = 20 units

Quick Example

Vial: 10 mg
Liquid added: 2 mL

10 ÷ 2 = 5 mg/mL
5 mg/mL = 5000 mcg/mL

If dose = 1 mg (1000 mcg):

1000 ÷ 5000 = 0.2 mL
0.2 mL = 20 units

Important Handling Tips

• Avoid shaking peptides.
• Always use sterile technique.
• Store reconstituted peptide refrigerated.

For additional peptide handling guides visit the Peptide Supply blog.

Research Use Only.